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KMID : 0604520160420030211
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Journal of the Society of Cosmetic Scientists of Korea
2016 Volume.42 No. 3 p.211 ~ p.216
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Evaluating the Micronucleus Induction Potential for the Genotoxicity Assay Using the Human Skin Model, KeraSkinTM
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Lee Su-Hyon
Jung Haeng-Sun
Kim Seol-Yeong
Kim Hye-Soo
Lim Kyung-Min
Chung Young-Shin
Choe Tae-Boo
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Abstract
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Micronucleus test is genotoxicity assay for detection of micronuclei in the cytoplasm of interphase cells. The reduction and replacement of in vivo toxicity testing on animals require the development of in vitro models to predict the genotoxicity or other tests for cosmetic products. In this study, we evaluated a genotoxicity assay for topically applied chemicals using a three-dimensional human reconstructed skin model, KeraSkinTM. Two genotoxins, mitomycin C (MMC) and methyl methanesulfonate (MMS), induced significant dose-related increases in cytotoxicity and micronuclei induction in the skin model. In contrast, two non-genotoxins, 4-nitrophenol (4-NP) and trichloroethylene (TCE), induced cytotoxicity but not micronucleus formation. In conclusion, micronucleus test using human skin model may be useful for predicting in vitro genotoxic potentials of cosmetic products.
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KEYWORD
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reconstructed human epidermis, KeraSkinTM, genotoxicity, micronucleustest
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